People » Jonathan Caranto

  • Roles of nitric oxide in microbial physiology
  • Metalloenzymology – intermediate trapping and mechanism
  • Natural product biosynthesis
  • Physiological roles of bacterial natural products

Our current arsenal of antibiotics may soon be obsolete with the rise of antibiotic-resistant bacteria. One strategy to combat these bacteria is to discover new antibiotics. Natural products (NPs) produced by bacteria are valuable sources of antibiotics as well as anti-fungal and anti-cancer agents. To provide new antibiotics and other drug candidates, our lab, led by Dr. Caranto, studies the biosynthesis of bacterial NPs. We use interdisciplinary approaches to achieve several goals: 1) discover NPs and their biosynthetic pathways using a combination of microbiology and bioinformatics techniques, 2) test enzymes from these pathways for new biological activities, and 3) elucidate the mechanisms of these enzymes using millisecond-mixing techniques and spectroscopies from the bioinorganic toolkit.

The estimated number of NPs that remain to be discovered is astronomical. To focus our research, we will study NPs that require nitric oxide (NO) for their formation. This gaseous molecule has well-known functions in human physiology, but its role in bacteria outside of energy transduction is unclear. By discovering new NO-dependent NPs and enzyme activities, we will provide insight into new physiological roles for NO in bacteria. In the future, we will expand our work to study the physiological roles of bacterial NPs in their native environments.

  1. Vilbert, A.C.; Caranto, J.D.; Lancaster, K.M. The Lysine Cross-Link to Heme P460 Obviates NO-Dependent Histidine-Dissociation from Nitrosomonas europaea Cytochrome P460 {FeNO}7. Sci. 2018, Advance Article.
  2. Weitz, A.C; Giri, N.; Caranto, J.D.; Kurtz, D.M., Jr.; Bominaar, E.L.; Hendrich, M.P. Spectroscopy and DFT calculations of a flavo-diiron enzyme implicate new diiron site structures, Am. Chem. Soc. 2017, 139, 12009–12019.
  3. Caranto, J.D.; Lancaster, K.M. Nitric oxide is an obligate bacterial nitrification intermediate produced by hydroxylamine oxidoreductase, Natl. Acad. Sci. U.S.A. 2017, 114, 8217–8222.
  4. Caranto, J.D; Vilbert, A.C.; Lancaster, K.M. Nitrosomonas europaea cytochrome P460 is a direct link between nitrification and nitrous oxide emission, Natl. Acad. Sci. U.S.A. 2016, 113, 14704–14709.
  5. Caranto, J.D.; Weitz, A.; Giri, N.; Hendrich, M.P.; Kurtz, D.M., Jr. A diferrous-dinitrosyl intermediate in the N2O-generating pathway of a deflavinated flavo-diiron protein, Biochemistry 2014, 53, 5631–5637.
  6. Caranto, J.D.; Weitz, A.; Hendrich, M.P.; Kurtz, D.M., Jr. The nitric oxide reductase mechanism of flavo-diiron protein: Identification of active-site intermediates and products, Am. Chem. Soc. 2014, 136, 7981–7992.
  7. Fang, H.; Caranto, J.D.; Mendoza, R.; Taylor A.B.; Hart, P.J.; Kurtz, D.M., Jr. Histidine ligand variants of a flavo-diiron protein: effects on structure and activities, Biol. Inorg. Chem. 2012, 17, 1231-1239.
  8. Caranto, J.D.; Gebhardt, L.L.; MacGowan, C.E.; Limberger, R.J.; Kurtz, D.M., Jr. Treponema denticola superoxide reductase: In vivo role, in vitro reactivities and a novel [Fe(Cys)4] site, Biochemistry, 2012, 51, 5601-5610.
  9. Hayashi, T.; Caranto, J.D.; Matsumara, H.; Kurtz, D.M. Jr.; Moenne-Loccoz, P. Vibrational analysis of mononitrosyl complexes in hemerythrin and flavodiiron proteins: relevance to detoxifying NO reductase, Am. Chem. Soc. 2012, 134, 6878-6884.
  10. Hayashi, T.; Caranto, J.D.; Wampler, D.A.; Kurtz, D.M. Jr.; Moenne-Loccoz, P. Insights into the nitric oxide reductase mechanism of flavo-diiron proteins from flavin-free enzyme, Biochemistry 2010, 49, 7040-7049.